EB1N-63高分断小型断路器:mobitec的巨大芽孢杆菌表达系统

更新时间：2026-09-27 04:25:20

EB1N-63高分断小型断路器

EB1N-63高分断小型断路器系列漏电断路器适用于交流50Hz、额定电压400V及单相230V，额定电流至63A的线路中，具有漏电触电、过载、短路等保护功能，还可以根据用户的需要增加过压保护功能。主要用于建筑照明和配电系统的保护。本产品也可根据用户要求，增加欠压、过压保护功能。mobitec的巨大芽孢杆菌表达系统SWP-D823昌晖双路温度显示仪现货促销 X-VIVO造血细胞无血清培养基多路巡检控制仪昌晖仪表酵母裂解酶（现货促销）2013北京KB-TC冷热冲击试验箱

hp-Vector表达系统megaterium芽孢杆菌
hp-Vector Expression Systems for Bacillus megaterium
Features:
High performance vectors with optimized sequence
Protein yield up to 10 times enhanced compared to protein expression with basic plasmid
Plasmids with established MCS
Encoding C- or N-terminal His-tag for versatile purification (native, 6xHis-tag)
Secretion with LipA or YocH signal peptide up to 9 fold increased
optimized high performance (hp) vectors for Bacillus megaterium!
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable to most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence and a 6xHis-tag.

With our new hp-vectors yields up to 10-fold higher compared to the basic plasmids are possible. All plasmids have established multiple cloning sites (MCS) for versatile cloning. Furthermore we offer vectors encoding C- or N-terminal His-tags for easy purification. The protein secretion with LipA or YocH signal peptides is up to nine-fold increased. Induction of protein expression of all vectors is achieved by the tightly regulated and efficiently inducible xylose operon.
Versatile system with a wide range of vectors
The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small- and large-scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.
B. megaterium protoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320, developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.
Order Information, shipping and storage
Please click on the respective order # for further details.
order# description amoun（订购货号信息）

Bacillus megaterium protoplasts ready for transformation (strain WH320;1)
Material is sufficient for 4 transformations plus control experiment. 5 x 500 μl
BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 μl
BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg
BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg
BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg
BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg
BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg

shipped at RT, protoplasts shipped on dry ice
store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C
All vectors are E. coli / B. megaterium shuttle vectors
Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.
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